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KMID : 0379520040200030205
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한국독성학회지
2004 Volume.20 No. 3 p.205 ~ p.211
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Endocrinic Effects of Toxaphene and Chlordane in Human Hepatoma Cell (HepG2 Cell) Transfected with Estrogen Receptor and Luciferase Reporter Gene
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Kim Kyung-Bae
Jung Ji-Won
Yang Se-Ran
Kang Kyung-Sun
Lee Yong-Soon
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Abstract
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Concern that some chemicals in our environment may affect human health by disrupt-ing normal endocrine function has prompted a research on interactions of environmental contaminants with steroid hormone receptor. Toxaphene and chlordane are among the 12 persistent organic pollutants identified by the United Nations Environment Programme as requiring urgent attention. We compared the estrogenic activity of two organochlorine pesticides, toxaphene and chlordane, at estrogen receptor a (ERalpha) and estrogen receptor beta (ERbeta). Human hepatoma cells (HepG2) were transiently transfected with rat ERalpha or ERbeta plus an estrogen-responsive complement C3-luciferase (C3-Luc) reporter gene. After transfection, cells were treated with various concentrations of toxaphene and chlordane to investigate agonism or antagonism of these chemicals. Both toxaphene and chlordane were potent agonists in HepG2 cells for ERalpha. In contrast, these chemicals had a minimal agonist activity with ERbeta and almost abolished 17beta-estradiol-induced ERbeta-mediated activity. Therefore, toxaphene and chlordane behaved as an ERalpha agonist and an ERbeta antagonist with estrogen-responsive reporter plasmid C3-Luc, and exposure to these organochlorine pesticides could have a crictical effect on normal endocrine function.
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KEYWORD
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Endocrine disruptor, Toxaphene, Chlordane, Estrogen receptors, ER{alpha}, ER{beta}, agonist, antagonist
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